This study demonstrated that the combination of EstroG-100^® and GABA provided early and progressive improvements in menopausal symptoms. Significant reductions in anxiety and stress were observed following the first intake (Day 0), while improvements in headache and mood changes were observed by Day 3. After 5 weeks of supplementation, significant improvements were observed in overall menopausal symptoms (GCS), mood state (POMS), sleep quality (PSQI), sexual function, and depressive symptoms. These findings suggest that EstroG-100^® combined with GABA may provide comprehensive support for both physical and psychological menopausal symptoms, contributing to improved overall quality of life.