EstroG-100^® significantly alleviated menopausal symptoms following 12 weeks of supplementation, with improvements in quality of life, sexual function, sleep quality, mood, psychological well-being, and bone health. The observed increase in serum serotonin levels, together with improvements in mood- and sleep-related outcomes, supports the involvement of a neuroendocrine regulatory mechanism. Importantly, these benefits were achieved without significant alterations in circulating hormone levels or other endocrine and metabolic biomarkers, suggesting that EstroG-100^® exerts its clinical effects without inducing systemic hormonal perturbation. These findings support EstroG-100^® as a safe and effective non-hormonal dietary intervention for the comprehensive management of menopausal symptoms.